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iasys optical biosensor  (Fisons Instruments Inc)

 
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    Fisons Instruments Inc iasys optical biosensor
    Iasys Optical Biosensor, supplied by Fisons Instruments Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iasys optical biosensor/product/Fisons Instruments Inc
    Average 90 stars, based on 1 article reviews
    iasys optical biosensor - by Bioz Stars, 2026-03
    90/100 stars

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    Affinities of TcTS-VHH complexes (IAsys biosensor analysis).

    Journal: PLoS ONE

    Article Title: Relevance of the Diversity among Members of the Trypanosoma Cruzi Trans-Sialidase Family Analyzed with Camelids Single-Domain Antibodies

    doi: 10.1371/journal.pone.0003524

    Figure Lengend Snippet: Affinities of TcTS-VHH complexes (IAsys biosensor analysis).

    Article Snippet: Kinetic analyses of the interactions were determined with IAsys Biosensor instrument (ThermoLabSystems).

    Techniques:

    Synthetic peptide (SP) specifically binds ODN2006. ( A ) Binding curves generated by the IAsys biosensor. Biotinylated ODN2006 was immobilized on the surface of a streptavidin-coated cuvette. A series of concentration of SP (3.1, 6.3, 12.5, 25 or 50 μM) or unrelated peptide (URP) (50 μM) were added separately into the cuvette to generate the binding curves. Data were analyzed using FASTplot, and the dissociation equilibrium constant (K D ) of SP was calculated using FASTfit; ( B ) Binding curves generated by the Biacore biosensor. Biotinylated ODN2006 was immobilized on a streptavidin-coated chip, and then a series of concentrations of SP (0.63, 1.25, 2.5, 5 or 10 μM) or URP (10 μM) were loaded separately to generate the binding curves. Data analysis and K D calculation were performed using the BIAevaluation software.

    Journal: International Journal of Molecular Sciences

    Article Title: Synthetic Human TLR9-LRR11 Peptide Attenuates TLR9 Signaling by Binding to and thus Decreasing Internalization of CpG Oligodeoxynucleotides

    doi: 10.3390/ijms17020242

    Figure Lengend Snippet: Synthetic peptide (SP) specifically binds ODN2006. ( A ) Binding curves generated by the IAsys biosensor. Biotinylated ODN2006 was immobilized on the surface of a streptavidin-coated cuvette. A series of concentration of SP (3.1, 6.3, 12.5, 25 or 50 μM) or unrelated peptide (URP) (50 μM) were added separately into the cuvette to generate the binding curves. Data were analyzed using FASTplot, and the dissociation equilibrium constant (K D ) of SP was calculated using FASTfit; ( B ) Binding curves generated by the Biacore biosensor. Biotinylated ODN2006 was immobilized on a streptavidin-coated chip, and then a series of concentrations of SP (0.63, 1.25, 2.5, 5 or 10 μM) or URP (10 μM) were loaded separately to generate the binding curves. Data analysis and K D calculation were performed using the BIAevaluation software.

    Article Snippet: The affinity assay was firstly carried out using an IAsys Plus affinity biosensor (Thermo Labsystem, Altrincham, Cheshire, UK), according to our previous report [ , ].

    Techniques: Binding Assay, Generated, Concentration Assay, Software